The Instrumentation Core:
The Mississippi INBRE project supports the Genomics Facility located at the University of Mississippi Medical Center, the Imaging Facility located at The University of Southern Mississippi, and the Proteomics Core located at Mississippi State University. These facilities are available to all Mississippi scientists and students at no cost thus providing access to high cost equipment that promotes biomedical research in the State.
The Genomics Facility (University of Mississippi Medical Center) is a training/research facility which has seen extensive use by faculty and students. The equipment most actively used are the Agilent bioanalyzer (for DNA and RNA analyses), the ScanArray Express scanner (microarray analysis), and the adjoining analytical computer and programs. Although the facility is primarily a microarray analysis facility, it also has a Multiporobe IIex robotic liquid handling system that can be programmed for high throughput assays or procedures. The facility can also make DNA or protein microarrays with a Packard Piezoelectric Biochip arrayer. Investigators who cannot easily visit the facility may send microarray slides to be scanned and analyzed
The Imaging Facility (University of Southern Mississippi) has seen extensive use by PUI researchers and other Mississippi scientists. The Zeiss Confocal Laser Scanning Microscope (CLSM) and the Synthetic Genetic Array (SGA) Robot are the most frequently used equipment. Additional equipment includes: Coulter Multisizer Counter, Chemidoc XRS System, Singer MSM Tetrad Dissector, Nanodrop ND-1000, Typhoon 9400 Phosphoimager, Genepix 4200A Microarray Scanner, Nikon Eclipse 80i Fluorescent Microscope and Agilent Bioanalyzer 2100.
The Proteomics Core (Mississippi State University). This core is a new addition to the MS-INBRE project and represents a partnership with the Life Sciences Biotechnology Institute. Major instrumentation includes: ThermoFinnigan Proteome X Workstations, Robotic Bio-Rad Proteome Works Spot Cutter, Nano 2D Lc-iTRAQ, ABI 4700 Proteomics Analyzer, and miscellaneous electrophoretic and imaging instrumentation.
